Identification of a novel miR-206-Notch3 pathway regulating mouse myoblasts proliferation

Zengkai Zhang; Yujun Chen; Bojiang Li; Yilong Yao; Aiwen Jiang; Wei Wei; Honglin Liu; Wangjun Wu

doi:10.1016/j.gene.2019.01.045

Identification of a novel miR-206-Notch3 pathway regulating mouse myoblasts proliferation

Gene. 2019 May 5:695:57-64. doi: 10.1016/j.gene.2019.01.045. Epub 2019 Feb 11.

Authors

Zengkai Zhang¹, Yujun Chen², Bojiang Li³, Yilong Yao⁴, Aiwen Jiang⁵, Wei Wei⁶, Honglin Liu⁷, Wangjun Wu⁸

Affiliations

¹ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].
² Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].
³ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
⁴ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
⁵ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].
⁶ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].
⁷ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].
⁸ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].

PMID: 30763672
DOI: 10.1016/j.gene.2019.01.045

Abstract

MicroRNAs (miRNAs) are endogenous short non-coding RNAs and exert their function by targeting mRNAs of genes. miRNA-206 (miR-206) is exclusively expressed in adult skeletal muscles and plays an important role in myogenesis. However, the regulatory mechanisms of miR-206 in myoblasts proliferation and differentiation are still limited. In this study, we validated that Notch3 is a direct target gene of mouse miR-206 using dual-luciferase reporter assay and miR-206-overexpressed experiments. Furthermore, we demonstrated that the negative effect of overexpression of miR-206 and knockdown of Notch3 on C2C12 cells proliferation, and found that the effect was produced by controlling the transition of G0/G1 and S phase in C2C12 cell cycle. In summary, our results provide direct evidences that miR-206 regulates skeletal muscle cells proliferation and cell cycle arrest partly by targeting the Notch3 gene, which strengthens our understanding of regulatory mechanisms on miR-206 in skeletal muscle growth and development.

Keywords: Cell cycle; Cell proliferation; Notch3; Skeletal muscle; miR-206.

MeSH terms

Animals
Cell Differentiation / genetics
Cell Line
Cell Proliferation / genetics*
Mice
MicroRNAs / genetics*
Muscle Development / genetics*
Muscle, Skeletal / growth & development
Myoblasts / cytology
Myoblasts / metabolism
Receptor, Notch3 / genetics*
Signal Transduction

Substances

MicroRNAs
Mirn206 microRNA, mouse
Notch3 protein, mouse
Receptor, Notch3