Detection of α-Synuclein in Biological Samples Using Mass Spectrometry

Ann Brinkmalm; Annika Öhrfelt; Payel Bhattacharjee; Henrik Zetterberg

doi:10.1007/978-1-4939-9124-2_16

Detection of α-Synuclein in Biological Samples Using Mass Spectrometry

Methods Mol Biol. 2019:1948:209-220. doi: 10.1007/978-1-4939-9124-2_16.

Authors

Ann Brinkmalm¹, Annika Öhrfelt¹, Payel Bhattacharjee¹, Henrik Zetterberg^{2

3

4

5}

Affiliations

¹ Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at the University of Gothenburg, Mölndal, Sweden.
² Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at the University of Gothenburg, Mölndal, Sweden. [email protected].
³ Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Mölndal, Sweden. [email protected].
⁴ Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London, UK. [email protected].
⁵ UK Dementia Research Institute at UCL, London, UK. [email protected].

PMID: 30771180
DOI: 10.1007/978-1-4939-9124-2_16

Abstract

Here we describe a method using mass spectrometry to characterize and quantify immuno-enriched α-synuclein forms from biochemically fractionated brain tissue.

Keywords: Brain; CSF; Mass spectrometry; Parkinson’s disease; α-Synuclein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brain / metabolism
Chromatography, Liquid
Databases, Factual
Immunoprecipitation
Isotope Labeling
Mass Spectrometry* / methods
Parkinson Disease / metabolism
Parkinson Disease / pathology
Workflow
alpha-Synuclein / cerebrospinal fluid
alpha-Synuclein / chemistry*
alpha-Synuclein / metabolism

Substances

alpha-Synuclein