Abstract
The use of viral vector-mediated α-synuclein-overexpressing rodent models for Parkinson's disease has become increasingly accepted to study associated pathology and to use as a platform for therapeutic efficacy testing. We here describe methods to generate such models and how to analyze them by behavioral assessments, histological investigations, and examination of nigrostriatal dopaminergic function.
Keywords:
Dopamine; Mouse model; Neurodegeneration; Parkinson’s disease; Rat model; Substantia nigra.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Behavior, Animal
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Biomarkers
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Cell Count
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Chromatography, High Pressure Liquid
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Disease Models, Animal*
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Dopamine / metabolism
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Dopaminergic Neurons / metabolism
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Dopaminergic Neurons / pathology
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Genetic Vectors / genetics*
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Immunohistochemistry
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Parkinson Disease / etiology*
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Parkinson Disease / metabolism*
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Parkinson Disease / pathology
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Rats
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Rodentia
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Stereotaxic Techniques
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Substantia Nigra / metabolism
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Substantia Nigra / pathology
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Viruses / genetics*