Optimizing biomethane production of mesophilic chicken manure and sheep manure digestion: Mono-digestion and co-digestion kinetic investigation, autofluorescence analysis and microbial community assessment

Optimization of mesophilic methane production from Chicken manure (CM) and Sheep manure (SM) at total solid (TS) of 8% and 1.6% were obtained by sequence tests in mono-digestion. However, the positive synergy of co-digestion with an optimum CM/SM of 2.5 (310 mLCH₄/gVS_added) resulted in a high hydrolytic capacity and methane production. The modified Gompertz model (R² > 0.98) and modified Aiba model (R² > 0.88) illustrated co-digestion significantly improved the methane generation rate with strong ammonia tolerance. Dissolved Organic Matter (DOM) variation in response to the metabolic rate of microbial community illustrated that the SMP-like and protein-like components half-split by EEM-PARAFAC were significantly negative corresponded to bio-methane production. Moreover, the canonical correlation analysis (CCA) resulted a significant difference between the substrate and DOM composition. Potential functional metabolic illustrated statistically significance difference between mono and co-digestion, however, Methanosaeta and Syntrophobacter predominated the syntrophic methanogenesis. The constructed complex metabolic cooperation caused the co-digestion stable and high efficiency.