Emergence of the East-Central-South-African genotype of Chikungunya virus in Brazil and the city of Rio de Janeiro may have occurred years before surveillance detection

Sci Rep. 2019 Feb 26;9(1):2760. doi: 10.1038/s41598-019-39406-9.

Abstract

Brazil, which is hyperendemic for dengue virus (DENV), has had recent Zika (ZIKV) and (CHIKV) Chikungunya virus outbreaks. Since March 2016, CHIKV is the arbovirus infection most frequently diagnosed in Rio de Janeiro. In the analysis of 1835 syndromic patients, screened by real time RT-PCR, 56.4% of the cases were attributed to CHIKV, 29.6% to ZIKV, and 14.1% to DENV-4. Sequence analyses of CHIKV from sixteen samples revealed that the East-Central-South-African (ECSA) genotype of CHIKV has been circulating in Brazil since 2013 [95% bayesian credible interval (BCI): 03/2012-10/2013], almost a year before it was detected by arbovirus surveillance program. Brazilian cases are related to Central African Republic sequences from 1980's. To the best of our knowledge, given the available sequence published here and elsewhere, the ECSA genotype was likely introduced to Rio de Janeiro early on 2014 (02/2014; BCI: 07/2013-08/2014) through a single event, after primary circulation in the Bahia state at the Northestern Brazil in the previous year. The observation that the ECSA genotype of CHIKV was circulating undetected underscores the need for improvements in molecular methods for viral surveillance.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bayes Theorem
  • Brazil / epidemiology
  • Chikungunya Fever / diagnosis*
  • Chikungunya Fever / epidemiology
  • Chikungunya Fever / virology
  • Chikungunya virus / classification
  • Chikungunya virus / genetics*
  • Chikungunya virus / isolation & purification
  • Genotype
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Phylogeny
  • RNA, Viral / chemistry
  • RNA, Viral / metabolism
  • Sequence Analysis, RNA

Substances

  • RNA, Viral