Solid-phase techniques in blood transfusion serology

Crit Rev Clin Lab Sci. 1986;22(4):317-42. doi: 10.3109/10408368509165789.

Abstract

For nearly a century, erythrocyte agglutination has persisted as the most widely used method for the demonstration of antigen-antibody reaction in immunohematology. So far, no other system has been developed which can match its simplicity, versatility, and general reliability. The major disadvantage of agglutination reactions is the lack of an objective endpoint, which has severely hindered attempts to automate routine pretransfusion tests. To overcome this problem, we have designed a series of solid-phase assays for ABO and Rh grouping, antibody screening, compatibility, and hepatitis tests. Each of these solid-phase assays shares a common endpoint of red cell adherence, which is easily interpreted visually or spectrophotometrically. Computer interface permits the automatic interpretation and recording of results. We believe this solid-phase system should finally bring the blood bank laboratory into the age of automation.

Publication types

  • Review

MeSH terms

  • ABO Blood-Group System / immunology
  • Antibodies, Anti-Idiotypic / immunology
  • Antibodies, Monoclonal
  • Blood Grouping and Crossmatching / methods*
  • Blood Platelets / immunology
  • Blood Transfusion*
  • Hemagglutination Tests / methods*
  • Hepatitis B Surface Antigens / analysis
  • Humans
  • Immune Adherence Reaction
  • Isoantibodies / analysis
  • Rh-Hr Blood-Group System / immunology
  • Saliva / immunology

Substances

  • ABO Blood-Group System
  • Antibodies, Anti-Idiotypic
  • Antibodies, Monoclonal
  • Hepatitis B Surface Antigens
  • Isoantibodies
  • Rh-Hr Blood-Group System