Objective:To observe the effect of 18β-sodium glycyrrhetinic acid(18β-SGA) on the expression of TNF-α in nasal mucosa of rats with allergic rhinitis（AR）, and explore the intervention mechanism of 18β-SGA on AR. Method:One hundred and six SPF-level Wistar rats were randomly divided into control group, AR group, budesonide group, 18β-SGA low dose group and high dose group. After the AR rat model was constructed by ovalbumin, the rats were given drug intervention and sacrificed after 2 and 4 weeks of intervention. The nasal mucosa of the rats was taken for immunohistochemical staining, RT-qPCR and Western-blotting to localize and quantify the expression of TNF-α. Result:By immunohistochemistry, Western-blotting and RT-PCR, TNF-α was mainly found in the columnar epithelium, vascular endothelium, glandular and some inflammatory cytoplasm of nasal mucosa. And the expression of TNF-α in the nasal mucosa of AR rats was significantly increased than the normal group at the protein and mRNA levels (P<0.01). After intervention with different doses of 18β-SGA, the expression of TNF-α was significantly decreased (P<0.01), especially after 4 weeks of 18β-SGA low dose group(P<0.01). Conclusion:Different doses of 18β-SGA have therapeutic effects on AR, and its mechanism of action may be related to the inhibition of TNF-α expression.
目的：观察18β-甘草次酸钠（18β-SGA）对变应性鼻炎（AR）大鼠鼻黏膜肿瘤坏死因子-α（TNF-α）表达的影响，探讨18β-SGA对AR的干预机制。方法：106只SPF级Wistar大鼠随机分为空白对照组、AR组、布地奈德组、18β-SGA低剂量组和18β-SGA高剂量组。经卵清蛋白构建AR大鼠模型后给予药物干预，各组大鼠分别在干预2周和4周后处死，取大鼠鼻黏膜行免疫组织化学染色、RT-PCR和免疫蛋白印迹定位、定量大鼠鼻黏膜中TNF-α表达。结果：经免疫组织化学、免疫印迹及RT-PCR发现，与空白对照组相比，AR组大鼠鼻黏膜中TNF-α高表达于鼻黏膜纤毛柱状上皮、血管内皮、腺体及部分炎症细胞胞质中（P<0.01）。经不同剂量的18β-SGA干预后，TNF-α表达量明显下降（P<0.01），尤以18β-SGA低剂量组干预4周后效果最明显（P<0.01）。结论：不同剂量的18β-SGA对AR有治疗作用，且其发挥作用的机制与抑制TNF-α的高表达有关。.
Keywords: rhinitis, allergic; 18β-sodium glycyrrhetinic acid; tumor necrosisi factor-α.
Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.