Microfluidic Device Directly Fabricated on Screen-Printed Electrodes for Ultrasensitive Electrochemical Sensing of PSA

Shouhui Chen; Zhihua Wang; Xinyuan Cui; Linlei Jiang; Yuee Zhi; Xianting Ding; Zhihong Nie; Pei Zhou; Daxiang Cui

doi:10.1186/s11671-019-2857-6

Microfluidic Device Directly Fabricated on Screen-Printed Electrodes for Ultrasensitive Electrochemical Sensing of PSA

Nanoscale Res Lett. 2019 Feb 28;14(1):71. doi: 10.1186/s11671-019-2857-6.

Authors

Shouhui Chen¹, Zhihua Wang², Xinyuan Cui³, Linlei Jiang¹, Yuee Zhi¹, Xianting Ding⁴, Zhihong Nie⁵, Pei Zhou⁶, Daxiang Cui^{7

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Affiliations

¹ Center of Food Safety Engineering and Technology Research, Shanghai, Key Laboratory of Urban Agriculture, Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
² Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
³ Department of Medical Imaging, Second Clinical College of Chongqing Medical University, No.74 Linjiang Road, Yuzhong District, Chongqing, 400016, China.
⁴ Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
⁵ Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA.
⁶ Center of Food Safety Engineering and Technology Research, Shanghai, Key Laboratory of Urban Agriculture, Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. [email protected].
⁷ Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. [email protected].
⁸ National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. [email protected].

Abstract

How to fabricate scale low-cost microfluidic device for detection of biomarkers owns a great requirement. Herein, it is for the first time reported that a new microfluidic device based on bonding polydimethylsiloxane microfluidic channels onto the substrate of a screen-printed electrode with coating glass solution was fabricated for electrochemical sensing of prostate-specific antigen (PSA). Compared to traditional microfabrication processes, this method is simple, fast, low cost, and also suitable for mass production. The prepared screen-printed electrode-based microfluidic device (CASPE-MFD) was used for the detection of the PSA in human serum. The prepared CASPE-MFD had a detection limit of 0.84 pg/mL (25.8 fM) and a good linearity with PSA concentration ranging from 0.001 to 10 ng/mL, which showed a great promise platform toward the development of miniaturized, low-cost electrochemical microfluidic device for use in human health, environmental monitoring, and other applications.

Keywords: Detection; Electrochemical sensor; Microfluidic devices; PSA; Screen-printed electrode.

Abstract

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