Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System

Sueli Marques; David van Bruggen; Gonçalo Castelo-Branco

doi:10.1007/978-1-4939-9072-6_1

Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System

Methods Mol Biol. 2019:1936:1-21. doi: 10.1007/978-1-4939-9072-6_1.

Authors

Sueli Marques¹, David van Bruggen¹, Gonçalo Castelo-Branco^{2

3}

Affiliations

¹ Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
² Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. [email protected].
³ Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, Stockholm, Sweden. [email protected].

PMID: 30820890
DOI: 10.1007/978-1-4939-9072-6_1

Abstract

Single-cell RNA sequencing has emerged as a powerful technique for the identification of distinct cell states/populations in complex tissues. We have recently used this technology to investigate heterogeneity of cells of the oligodendrocyte lineage in the mouse central nervous system. In this chapter, we describe methods to perform single-cell RNA sequencing on this glial cell lineage, and discuss experimental and computational approaches to explore the potential and to tackle hurdles associated with this technology.

Keywords: Central nervous system; Myelin; Oligodendrocyte; RNA sequencing; Single-cell; Transcriptomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Cell Lineage
Central Nervous System / chemistry
Central Nervous System / cytology*
Gene Expression Profiling
Gene Expression Regulation
High-Throughput Nucleotide Sequencing
Mice
Oligodendroglia / chemistry
Oligodendroglia / cytology*
Sequence Analysis, RNA / methods*
Single-Cell Analysis / methods*