Choosing your (Friedel) mates wisely: grouping data sets to improve anomalous signal

Acta Crystallogr D Struct Biol. 2019 Feb 1;75(Pt 2):200-210. doi: 10.1107/S205979831801570X. Epub 2019 Jan 31.

Abstract

Single-wavelength anomalous diffraction (SAD) phasing from multiple crystals can be especially challenging in samples with weak anomalous signals and/or strong non-isomorphism. Here, advantage is taken of the combinatorial diversity possible in such experiments to study the relationship between merging statistics and downstream metrics of phasing signals. It is furthermore shown that a genetic algorithm (GA) can be used to optimize the grouping of data sets to enhance weak anomalous signals based on these merging statistics.

Keywords: anomalous scattering; merging; multi-crystal crystallography; serial crystallography; single-wavelength anomalous diffraction.

MeSH terms

  • Algorithms
  • Bacillus / chemistry
  • Bacterial Proteins / chemistry
  • Crystallization / methods
  • Crystallography, X-Ray / methods*
  • Data Collection / methods*
  • Models, Molecular
  • Protein Conformation
  • Sporosarcina / chemistry
  • Thermolysin / chemistry
  • Urease / chemistry

Substances

  • Bacterial Proteins
  • Thermolysin
  • Urease