Revealing mcr-1-positive ESBL-producing Escherichia coli strains among Enterobacteriaceae from food-producing animals (bovine, swine and poultry) and meat (bovine and swine), Portugal, 2010-2015

Lurdes Clemente; Vera Manageiro; Ivone Correia; Ana Amaro; Teresa Albuquerque; Patrícia Themudo; Eugénia Ferreira; Manuela Caniça

doi:10.1016/j.ijfoodmicro.2019.02.006

Revealing mcr-1-positive ESBL-producing Escherichia coli strains among Enterobacteriaceae from food-producing animals (bovine, swine and poultry) and meat (bovine and swine), Portugal, 2010-2015

Int J Food Microbiol. 2019 May 2:296:37-42. doi: 10.1016/j.ijfoodmicro.2019.02.006. Epub 2019 Feb 10.

Authors

Lurdes Clemente¹, Vera Manageiro², Ivone Correia¹, Ana Amaro¹, Teresa Albuquerque¹, Patrícia Themudo¹, Eugénia Ferreira², Manuela Caniça³

Affiliations

¹ INIAV - National Institute of Agrarian and Veterinary Research, Bacteriology and Micology Laboratory, Oeiras, Portugal.
² National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr Ricardo Jorge, Lisbon, Portugal; Centre for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, University of Oporto, Oporto, Portugal.
³ National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr Ricardo Jorge, Lisbon, Portugal; Centre for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, University of Oporto, Oporto, Portugal. Electronic address: [email protected].

PMID: 30844701
DOI: 10.1016/j.ijfoodmicro.2019.02.006

Abstract

We screened 1840 Enterobacteriaceae isolates from food-producing animals, meat, meat products and animal feed, for the detection of plasmid-mediated colistin resistance, during 2010-2015. The mcr-1 gene was detected in 8.0% (97/1206) Escherichia coli and in 0.47% (3/634) Salmonella enterica isolates, with a high number of mcr-1 positive E. coli isolates (45.7%) being extended-spectrum β-lactamase or plasmid-mediated AmpC β-lactamase co-producers. No mcr-2 gene was detected. Our findings highlight the spread of mcr-1 genes within a wide-ranging sample of food-producing animals and meat, in Portugal.

Keywords: ESBL; Enterobacteriaceae; Food-producing animals; MCR-1.9; Meat; PMAβ; mcr-1 emergence.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Cattle
Chickens / microbiology
Colistin / pharmacology
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli / metabolism
Escherichia coli Proteins / biosynthesis
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / isolation & purification
Meat / microbiology
Membrane Proteins / genetics*
Microbial Sensitivity Tests
Plasmids / genetics
Portugal
Poultry
Salmonella enterica / drug effects
Salmonella enterica / genetics*
Salmonella enterica / metabolism
Swine / microbiology*
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Bacterial Proteins
Escherichia coli Proteins
MCR-1 protein, E coli
Membrane Proteins
mcr-2 protein, E coli
AmpC beta-lactamases
beta-Lactamases
Colistin