Abstract
We identified 29 G-quadruplex binding proteins by affinity purification and quantitative LC-MS/MS. We demonstrated that the DEAD-box RNA helicases Dbp2, Ded1 and Mss116 preferentially bind to G-quadruplex nucleic acids in vitro and destabilize RNA quadruplexes, suggesting new potential roles for these helicases in disruption of quadruplex structures in RNA.
MeSH terms
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Adenosine Triphosphate / chemistry
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Adenosine Triphosphate / metabolism
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Chromatography, High Pressure Liquid
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DEAD-box RNA Helicases / chemistry*
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DEAD-box RNA Helicases / genetics
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DEAD-box RNA Helicases / metabolism
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G-Quadruplexes*
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Kinetics
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Protein Binding
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae Proteins / chemistry*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Tandem Mass Spectrometry
Substances
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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Adenosine Triphosphate
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DEAD-box RNA Helicases