Genetic Characterization of a bla VIM-24-Carrying IncP-7β Plasmid p1160-VIM and a bla VIM-4-Harboring Integrative and Conjugative Element Tn 6413 From Clinical Pseudomonas aeruginosa

Lijun Zeng; Zhe Zhan; Lingfei Hu; Xiaoyuan Jiang; Yanjun Zhang; Jiao Feng; Bo Gao; Yuee Zhao; Wenhui Yang; Huiying Yang; Zhe Yin; Dongsheng Zhou

doi:10.3389/fmicb.2019.00213

Genetic Characterization of a bla _VIM-24-Carrying IncP-7β Plasmid p1160-VIM and a bla _VIM-4-Harboring Integrative and Conjugative Element Tn 6413 From Clinical Pseudomonas aeruginosa

Front Microbiol. 2019 Feb 26:10:213. doi: 10.3389/fmicb.2019.00213. eCollection 2019.

Authors

Lijun Zeng^{1

2}, Zhe Zhan^{1

3}, Lingfei Hu¹, Xiaoyuan Jiang¹, Yanjun Zhang³, Jiao Feng¹, Bo Gao¹, Yuee Zhao¹, Wenhui Yang¹, Huiying Yang¹, Zhe Yin¹, Dongsheng Zhou¹

Affiliations

¹ State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
² Department of Clinical Laboratory, The 307th Hospital of the Chinese People's Liberation Army, Beijing, China.
³ Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.

Abstract

This study presents three novel integrons In1394, In1395, and In1443, three novel unit transposons Tn6392, Tn6393, and Tn6403, one novel conjugative element (ICE) Tn6413, and the first sequenced IncP-7 resistance plasmid p1160-VIM from clinical Pseudomonas aeruginosa. Detailed sequence comparison of p1160-VIM (carrying Tn6392 and Tn6393) and Tn6413 (carrying Tn6403) with related elements were performed. Tn6392, Tn6393, and Tn6403 were generated from integration of In1394 (carrying bla _VIM-24), In1395 and In1443 (carrying bla _VIM-4) into prototype Tn3-family unit transposons Tn5563, Tn1403, and Tn6346, respectively. To the best of our knowledge, this is the first report of a bla _VIM-24-carrying P. aeruginosa isolate.

Keywords: IncP-7 plasmid; Pseudomonas aeruginosa; blaVIM; integrative and conjugative element; unit transposon.