Generation of two transgene-free human iPSC lines from CD133+ cord blood cells

Estibaliz Arellano-Viera; Lorea Zabaleta; Julio Castaño; Garikoitz Azkona; Xonia Carvajal-Vergara; Alessandra Giorgetti

doi:10.1016/j.scr.2019.101410

Generation of two transgene-free human iPSC lines from CD133⁺ cord blood cells

Stem Cell Res. 2019 Apr:36:101410. doi: 10.1016/j.scr.2019.101410. Epub 2019 Feb 28.

Authors

Estibaliz Arellano-Viera¹, Lorea Zabaleta¹, Julio Castaño¹, Garikoitz Azkona¹, Xonia Carvajal-Vergara², Alessandra Giorgetti³

Affiliations

¹ Inbiomed Foundation, San Sebastian, Spain.
² Inbiomed Foundation, San Sebastian, Spain. Electronic address: [email protected].
³ Inbiomed Foundation, San Sebastian, Spain. Electronic address: [email protected].

PMID: 30878013
DOI: 10.1016/j.scr.2019.101410

Abstract

We have generated two human induced pluripotent stem cell (iPSC) lines from CD133⁺ cells isolated from umbilical cord blood (CB) of a female child using non-integrative Sendai virus. Here we describe the complete characterization of these iPSC lines: PRYDi-CB5 and PRYDi-CB40.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AC133 Antigen / genetics*
Animals
Cell Differentiation
Cell Line*
Cellular Reprogramming Techniques
Clone Cells
Fetal Blood / cytology
Genetic Markers
Humans
Induced Pluripotent Stem Cells*
Karyotype
Mice, Inbred NOD
Mice, SCID
Sendai virus

Substances

AC133 Antigen
Genetic Markers
PROM1 protein, human