An exploratory study of articular cartilage and subchondral bone reconstruction with bone marrow mesenchymal stem cells combined with porous tantalum/Bio-Gide collagen membrane in osteonecrosis of the femoral head

Baoyi Liu; Fan Yang; Xiaowei Wei; Xiuzhi Zhang; Yu Zhang; Benjie Wang; Ge Liu; Hui Xie; Jiahui Yang; Wei Wang; Kairong Qin; Dewei Zhao

doi:10.1016/j.msec.2019.02.072

An exploratory study of articular cartilage and subchondral bone reconstruction with bone marrow mesenchymal stem cells combined with porous tantalum/Bio-Gide collagen membrane in osteonecrosis of the femoral head

Mater Sci Eng C Mater Biol Appl. 2019 Jun:99:1123-1132. doi: 10.1016/j.msec.2019.02.072. Epub 2019 Feb 19.

Authors

Baoyi Liu¹, Fan Yang², Xiaowei Wei³, Xiuzhi Zhang³, Yu Zhang³, Benjie Wang³, Ge Liu³, Hui Xie³, Jiahui Yang³, Wei Wang³, Kairong Qin⁴, Dewei Zhao⁵

Affiliations

¹ Department of Orthopedics, Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian, Liaoning, China; Postdoctoral Workstation, Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian, Liaoning, China; First Hospital and Institute of Immunology, Jilin University, Changchun, Jilin, China.
² Department of Orthopedics, Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian, Liaoning, China; Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, China.
³ Department of Orthopedics, Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian, Liaoning, China.
⁴ Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, China.
⁵ Department of Orthopedics, Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian, Liaoning, China; Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, China. Electronic address: [email protected].

PMID: 30889646
DOI: 10.1016/j.msec.2019.02.072

Abstract

Osteonecrosis of the femoral head (ONFH) results in collapse of the femoral head and rapid destruction of the hip joint. The repair of post-collapse articular cartilage and subchondral bone is challenging. We interrupted the blood supply to the femoral head and established a full-thickness articular defect animal model after ONFH was determined via X-ray. Porous tantalum and a Bio-Gide collagen membrane, co-cultured with bone marrow mesenchymal stem cells (BMSCs) in vitro, were implanted into the defect zone to repair the full-thickness articular defect. Hyaline cartilage was detected on top of the tantalum near the edge of the defect 12 weeks post-operatively. Porous tantalum and a Bio-Gide collagen membrane with BMSCs may repair full-thickness articular defects if the blood supply can be reconstructed in the post-collapse stage of ONFH.

Keywords: Bio-Gide collagen membrane; Bone marrow stromal stem cells; Full-thickness articular cartilage defect; Osteonecrosis of femoral head; Porous tantalum.

MeSH terms

Animals
Cartilage, Articular / pathology*
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Cells, Cultured
Chondrocytes / cytology
Chondrocytes / drug effects
Chondrogenesis / drug effects
Coculture Techniques
Collagen / pharmacology*
Femur Head Necrosis / pathology
Femur Head Necrosis / therapy*
Membranes, Artificial*
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / ultrastructure
Osteogenesis / drug effects
Phenotype
Rabbits
Tantalum / pharmacology*

Substances

Bio-Gide
Membranes, Artificial
Tantalum
Collagen