Video-rate quantitative phase analysis by a DIC microscope using a polarization camera

Shuhei Shibata; Wataru Takano; Nathan Hagen; Masaru Matsuda; Yukitoshi Otani

doi:10.1364/BOE.10.001273

Video-rate quantitative phase analysis by a DIC microscope using a polarization camera

Biomed Opt Express. 2019 Feb 19;10(3):1273-1281. doi: 10.1364/BOE.10.001273. eCollection 2019 Mar 1.

Authors

Shuhei Shibata^{1

2}, Wataru Takano^{1

2}, Nathan Hagen^{1

2}, Masaru Matsuda³, Yukitoshi Otani^{1

2}

Affiliations

¹ Department of Optical Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan.
² Center for Optical Research and Education, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan.
³ Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi, Japan.

Abstract

This paper describes how to take advantage of the replacement of an intensity camera with a polarization camera in a standard differential interference contrast (DIC) microscope. Using a polarization camera enables snapshot quantitative phase analysis so that real-time imaging of living transparent tissues become possible. Using our method, we quantify the phase measurement accuracy using a phantom consisting of glass beads embedded in lacquer. In order to demonstrate these advantages, we image the pumping heart and blood flow in a living medaka egg.