Enumeration of the T4 positive and T8 positive lymphocytes in AIDS and related syndromes. Comparison of the immunogold and the immunofluorescence techniques

S Chollet-Martin; M A Gougerot-Pocidalo

doi:10.1016/0022-1759(86)90489-8

Enumeration of the T4 positive and T8 positive lymphocytes in AIDS and related syndromes. Comparison of the immunogold and the immunofluorescence techniques

J Immunol Methods. 1986 Jul 24;91(2):271-4. doi: 10.1016/0022-1759(86)90489-8.

Authors

S Chollet-Martin, M A Gougerot-Pocidalo

PMID: 3090151
DOI: 10.1016/0022-1759(86)90489-8

Abstract

Immunofluorescence and immunogold techniques yielded similar results when used to determine the T lymphocyte subsets (T4 and T8 positive cells) in patients with AIDS or AIDS-related complex and in healthy homosexual men seropositive for HTLV III/LAV antibody. In these pathological situations, the advantages of immunogold staining could render this technique useful in a clinical laboratory.

MeSH terms

Acquired Immunodeficiency Syndrome / immunology*
Antigens, Differentiation, T-Lymphocyte
Antigens, Surface / analysis*
Fluorescent Antibody Technique
Gold
Homosexuality
Humans
Immunologic Techniques
Leukocyte Count
Lymphatic Diseases / immunology*
Male
T-Lymphocytes / classification*
T-Lymphocytes / immunology
T-Lymphocytes / microbiology

Substances

Antigens, Differentiation, T-Lymphocyte
Antigens, Surface
Gold