Mysterious effects of olfactory pathway lesions on intestinal immunodeficiency targeting Peyer's patches: The first experimental study

Background: Although olfaction has been considered as important neuroimmunomodulatory foundation, there is no satisfying analytical information between neurohistomorphological features olfactory networks and intestinal immune system hardwares. We studied if the olfactory bulb lesions (OBL) may rely on histopathological features of intestinal lymphatic Peyer's patches in an animal model.

Methods: Thirty-two rats were grouped as control (Group I, n = 8), SHAM (Group II, n = 7) and OBL (Group III, n = 17) respectively; and followed eight weeks and animals were decapitated. The olfactory bulbs and intestines were extracted. Specimens stained with hematoxylin/eosin and GFAP methods and analyzed Stereologically to evaluate volume loss of olfactory bulbs and Peyer's patches volumes (PV) of intestines per cubic millimeter and compared with each other's statistically.

Results: The mean olfactory bulbs volumes were estimated as 3.65 ± 0.32/mm³ in group I, 3.12 ± 0.20/mm³ in group II and 2.21 ± 0.15/mm³ in group III (p < 0.0005 Group III vs. I and II). The mean of PV were estimated as; (9 ± 2) × 10⁶ µm³/cm³ in Group-I, (12 ± 3) × 10⁶ µm³/cm³ in Group-II; and (23 ± 4) × 10⁶ µm³/cm³ in group-III (p < 0.005 Group II vs. I, p < 0.0005 Group III vs. I-II).

Conclusions: OBL could rely on intestinal immunodeficiency causing by olfaction loss induced denervation injury of Peyer's patches.