Intestinal vitamin D receptor modulates lipid metabolism, adipose tissue inflammation and liver steatosis in obese mice

Biochim Biophys Acta Mol Basis Dis. 2019 Jun 1;1865(6):1567-1578. doi: 10.1016/j.bbadis.2019.03.007. Epub 2019 Mar 21.

Abstract

Objective: Hypovitaminosis D is common in the obese population and patients suffering from obesity-associated disorders such as type 2 diabetes and fatty liver disease, resulting in suggestions for vitamin D supplementation as a potential therapeutic option. However, the pathomechanistic contribution of the vitamin D-vitamin D receptor (VDR) axis to metabolic disorders is largely unknown.

Methods: We analyzed the pathophysiological role of global and intestinal VDR signaling in diet-induced obesity (DIO) using global Vdr-/- mice and mice re-expressing an intestine-specific human VDR transgene in the Vdr deficient background (Vdr-/- hTg).

Results: Vdr-/- mice were protected from DIO, hepatosteatosis and metabolic inflammation in adipose tissue and liver. Furthermore, Vdr-/- mice displayed a decreased adipose tissue lipoprotein lipase (LPL) activity and a reduced capacity to harvest triglycerides from the circulation. Intriguingly, all these phenotypes were partially reversed in Vdr-/- hTg animals. This clearly suggested an intestine-based VDR activity on systemic lipid homeostasis. Scrutinizing this hypothesis, we identified the potent LPL inhibitor angiopoietin-like 4 (Angptl4) as a novel transcriptional target of VDR.

Conclusion: Our study suggests a VDR-mediated metabolic cross-talk between gut and adipose tissue, which significantly contributes to systemic lipid homeostasis. These results have important implications for use of the intestinal VDR as a therapeutic target for obesity and associated disorders.

Keywords: Hepatic; Mouse model; Non-alcoholic fatty liver disease; Overweight.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism
  • Adipose Tissue / pathology
  • Adult
  • Aged
  • Angiopoietin-Like Protein 4 / genetics*
  • Angiopoietin-Like Protein 4 / metabolism
  • Animals
  • Cohort Studies
  • Fatty Liver / genetics*
  • Fatty Liver / metabolism
  • Fatty Liver / pathology
  • Female
  • Gene Expression Regulation
  • Humans
  • Inflammation
  • Intestinal Mucosa / metabolism*
  • Intestinal Mucosa / pathology
  • Lipid Metabolism / genetics
  • Lipoprotein Lipase / antagonists & inhibitors
  • Lipoprotein Lipase / genetics*
  • Lipoprotein Lipase / metabolism
  • Liver / metabolism*
  • Liver / pathology
  • Male
  • Mice
  • Mice, Obese
  • Mice, Transgenic
  • Middle Aged
  • Receptors, Calcitriol / deficiency
  • Receptors, Calcitriol / genetics*
  • Signal Transduction
  • Transcription, Genetic
  • Transgenes
  • Triglycerides / metabolism

Substances

  • Angiopoietin-Like Protein 4
  • Receptors, Calcitriol
  • Triglycerides
  • VDR protein, human
  • Lipoprotein Lipase