Limited preventive effect of prednisone on neuropsychiatric symptoms in murine systemic lupus erythematosus

Inflammopharmacology. 2019 Jun;27(3):511-520. doi: 10.1007/s10787-019-00587-4. Epub 2019 Mar 25.

Abstract

Objective: To investigate whether glucocorticoids, the hallmark medication for systemic lupus erythematosus (SLE), could prevent the development of neuropsychiatric SLE (NPSLE).

Methods: The protective effects of prednisone on NPSLE were tested using the open field, object recognition/placement, forced swim, tail suspension, and sucrose preference tests in MRL/lpr mice. Auto-antibody titres and the weight of lymph nodes were also measured.

Results: MRL/lpr mice exhibited mild depression at the age of 8 weeks before progressing with spatial cognitive impairment and severe depression-like behaviour at the age of 16 weeks. Treating MRL/lpr mice with prednisone (5 mg/kg) from the age of 8 weeks decreased anti-cardiolipin and anti-N-methyl-D-aspartate (NMDA) receptor antibody titres in the brain, reduced the weight of lymph nodes, and prolonged the floating latency in the forced swim test. However, prednisone (3 or 5 mg/kg) had no preventive effect on the development of spatial cognitive impairment and other depression-like behaviours in MRL/lpr mice. The dose of prednisone had a positive correlation with the floating latency in the forced swim test, while it offered no effects on all other behavioural tests.

Conclusion: Our results provide evidence that early treatment with prednisone had a limited effect on the development of neuropsychiatric symptoms in MRL/lpr mice. Further work is needed in other models beyond NPSLE in MRL/lpr mice before any definitive conclusions are made on the efficacy of prednisone in human NPSLE.

Keywords: Cognitive impairment; Depression; Glucocorticoid; Neuropsychiatric systemic lupus erythematosus; Prednisone.

MeSH terms

  • Animals
  • Brain / drug effects
  • Brain / metabolism
  • Cardiolipins / metabolism
  • Depression / metabolism
  • Disease Models, Animal
  • Female
  • Glucocorticoids / pharmacology
  • Lupus Vasculitis, Central Nervous System / drug therapy*
  • Lupus Vasculitis, Central Nervous System / metabolism
  • Lymph Nodes / metabolism
  • Mice
  • Mice, Inbred MRL lpr
  • Prednisone / pharmacology*
  • Protective Agents / pharmacology
  • Receptors, N-Methyl-D-Aspartate / metabolism

Substances

  • Cardiolipins
  • Glucocorticoids
  • Protective Agents
  • Receptors, N-Methyl-D-Aspartate
  • Prednisone