Abstract
We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody-fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / chemistry*
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Antibodies / metabolism
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Click Chemistry
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Molecular Structure
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Peptide Synthases / chemistry*
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Peptide Synthases / metabolism
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Protein Binding
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Recombinant Fusion Proteins / chemistry*
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Recombinant Fusion Proteins / metabolism
Substances
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Antibodies
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Recombinant Fusion Proteins
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Peptide Synthases
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tyrosyltubulin ligase