In this study, we designed an improved method to construct gene-deficient strain in non-conventional yeast Pichia pastoris. This method achieved high efficiency of gene deletion without disruption of NHEJ-related genes. The disruption efficiency of Och1 reached about 80%. This simple method will be a new useful molecular tool for P. pastoris.
Keywords: Gene disruption; Homologous recombination; Non-homologous end-joining; Pichia pastoris.
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