Impact of the Timing of Antibiotic Administration on Digestive Colonization with Carbapenemase-Producing Enterobacteriaceae in a Murine Model

Antimicrob Agents Chemother. 2019 May 24;63(6):e00360-19. doi: 10.1128/AAC.00360-19. Print 2019 Jun.

Abstract

While antibiotic use is a risk factor of carbapenemase-producing Enterobacteriaceae (CPE) acquisition, the importance of timing of antibiotic administration relative to CPE exposure remains unclear. In a murine model of gut colonization by New Delhi metallo-beta-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae, a single injection of clindamycin within at most 1 week before or after CPE exposure induced colonization persisting up to 100 days. The timing of antibiotic administration relative to CPE exposure may be relevant to infection control and antimicrobial stewardship approaches.

Keywords: Klebsiella pneumoniae; NDM-1; carbapenemase; gut microbiota; murine model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage*
  • Bacterial Proteins / metabolism*
  • Disease Models, Animal
  • Enterobacteriaceae / drug effects*
  • Enterobacteriaceae / metabolism
  • Enterobacteriaceae Infections / drug therapy*
  • Gastrointestinal Microbiome / drug effects*
  • Infection Control / methods
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / metabolism
  • Mice
  • Microbial Sensitivity Tests / methods
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactamases
  • beta-lactamase NDM-1
  • carbapenemase