miR-134 inhibits osteosarcoma cell invasion and metastasis through targeting MMP1 and MMP3 in vitro and in vivo

FEBS Lett. 2019 May;593(10):1089-1101. doi: 10.1002/1873-3468.13387. Epub 2019 May 8.

Abstract

miR-134 has been shown to be associated with angiogenesis and the progression of osteosarcoma. This study further assessed the effects of miR-134 expression on osteosarcoma cell migration, invasion, and metastasis in vitro and in a nude mouse xenograft model, exploring the underlying molecular events. Luciferase reporter assays revealed that miR-134 directly targets the 3'-UTRs of MMP1 and MMP3 to reduce their expression in osteosarcoma cells. In conclusion, overexpression of miR-134 suppresses osteosarcoma cell invasion and metastasis through the inhibition of MMP1 and MMP3 expression. We propose miR-134 as an attractive novel therapeutic target for the treatment of osteosarcoma.

Keywords: invasion; matrix metalloproteinases; metastasis; miR-134; osteosarcoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Bone Neoplasms / genetics*
  • Bone Neoplasms / metabolism
  • Bone Neoplasms / physiopathology
  • Cell Line, Tumor
  • Cell Movement
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Matrix Metalloproteinase 1 / genetics*
  • Matrix Metalloproteinase 3 / genetics*
  • Mice
  • Mice, Nude
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness
  • Osteosarcoma / genetics*
  • Osteosarcoma / metabolism
  • Osteosarcoma / physiopathology
  • Xenograft Model Antitumor Assays

Substances

  • 3' Untranslated Regions
  • MIRN134 microRNA, human
  • MicroRNAs
  • MMP3 protein, human
  • Matrix Metalloproteinase 3
  • MMP1 protein, human
  • Matrix Metalloproteinase 1