The different effects of viral and bacterial mimics maternal stimuli on ethology of hens and reproduction of their offspring

Environmental stimuli resulting from immunological stress can induce transgenerational phenotypic inheritance, but few similar studies are found in avian. Here, we challenged F0 hens with polyinosinic: polycytidylic acid [Poly(I: C)] and lipopolysaccharide (LPS) at 53 wk of age, and then investigated the ethology of the challenged hens. In the unchallenged F1 descendants, the egg quality at 23 wk of age and laying rate (LR) at different stages were measured. Mortality rate (MR) and the days of population LR reaching 50% (D50%LR) at 33 wk of age were also tested in F1 hens. Pearson correlation analysis was subsequently calculated between F1 peripheral blood lymphocytes transcriptome and LR (in L vs. C) and EW (in P vs. C), respectively. The results showed that the ethology and egg-laying variations of stimuli-challenged hens and their descendants could be affected by the 2 kinds of immune stimuli. Poly(I: C) was likely to increase LR, especially in the early laying period and advance the D50%LR in F1 hens. It also reduced the MR, albumen height, and Haugh units of the unchallenged offspring. Whereas LPS could induce a sickness behavior of the challenged F0 hens, it also reduced the LR of F1 hens throughout the study, prolonged the D50%LR, and faded the eggshell color. Correlation analysis showed that Poly(I: C) mainly affected EW, while LPS mainly influenced LR of F1 offspring. All findings in the present study were the first time to be revealed in laying chickens, suggesting the different effects of Poly(I: C) and LPS on chickens and their descendants, and laying the foundation for the study of the influence of maternal experience on offspring in avian.