Abstract
Abscisic acid (ABA) plays critical roles during plant growth and development in response to various stresses. Arabidopsis thaliana histone demethylases JUMONJI-C DOMAIN-CONTAINING PROTEIN 30 (JMJ30) and JMJ32 control ABA-mediated growth arrest during the post-germination stage (2-3 days after germination). However, the roles of JMJ30 and JMJ32 in ABA responses at later stages of plant development remain largely unknown. Here, we show that JMJ30 and JMJ32 mediate ABA responses during root development. In the presence of ABA, jmj30 jmj32 double mutants display longer primary roots than the wild type. Loss-of-function mutation in the SNF1-RELATED PROTEIN KINASE 2.8 (SnRK2.8) gene also led to a longer primary root phenotype in response to ABA. Analysis of JMJ30/JMJ32 and SnRK2.8 expression suggested that they act in the same pathway to mediate ABA responses during root elongation at the seedling stage. Our findings highlight the importance of the JMJ30/JMJ32-SnRK2.8 module at two different developmental stages.
Keywords:
; abscisic acid; JUMONJI; SNF1-RELATED PROTEIN KINASE 2.8.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / metabolism*
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Abscisic Acid / pharmacology
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Arabidopsis / enzymology*
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Arabidopsis / genetics
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Gene Expression Regulation, Plant / drug effects
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Jumonji Domain-Containing Histone Demethylases / genetics
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Jumonji Domain-Containing Histone Demethylases / metabolism*
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Mutation / genetics
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Plant Roots / drug effects
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Plant Roots / enzymology*
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Plant Roots / growth & development*
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Signal Transduction* / drug effects
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Stress, Physiological* / drug effects
Substances
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Arabidopsis Proteins
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Abscisic Acid
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JMJ30 protein, Arabidopsis
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JMJ32 protein, Arabidopsis
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Jumonji Domain-Containing Histone Demethylases
Grants and funding
This work was supported by grants from the Japan Science and Technology Agency ‘Precursory Research for Embryonic Science and Technology’ (JPMJPR15QA), JSPS KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (no. 16H01468, 18H04782), JSPS KAKENHI Grant-in-Aid for Scientific Research B (no. 18H02465), the NAIST foundation, and the Mishima Kaiun Foundation to N.Y.; and grants from the Mitsubishi Foundation, JSPS KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (no. 17H05843, 18H04839), JSPS KAKENHI Grant-in-Aid for Challenging Research (no. 18K19342), and JSPS KAKENHI Grant-in-Aid for Scientific Research A (no. 15H02405) to T.I.