Micro-Nano Bioactive Glass Particles Incorporated Porous Scaffold for Promoting Osteogenesis and Angiogenesis in vitro

Ting Tian; Weihan Xie; Wendong Gao; Gang Wang; Lei Zeng; Guohou Miao; Bo Lei; Zhanyi Lin; Xiaofeng Chen

doi:10.3389/fchem.2019.00186

Micro-Nano Bioactive Glass Particles Incorporated Porous Scaffold for Promoting Osteogenesis and Angiogenesis in vitro

Front Chem. 2019 Mar 29:7:186. doi: 10.3389/fchem.2019.00186. eCollection 2019.

Authors

Ting Tian^{1

2

3}, Weihan Xie^{2

3

4}, Wendong Gao^{2

3

4}, Gang Wang^{2

3

4}, Lei Zeng^{2

3

4}, Guohou Miao⁵, Bo Lei⁶, Zhanyi Lin^{1

7}, Xiaofeng Chen^{2

3

4}

Affiliations

¹ Guangzhou Higher Education Mega Center, School of Medicine, South China University of Technology, Guangzhou, China.
² Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China.
³ National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China.
⁴ Key Laboratory of Biomedical Materials and Engineering, South China University of Technology, Ministry of Education, Guangzhou, China.
⁵ Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China.
⁶ Instrument Analysis Center, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, China.
⁷ Department of Cardiology, Guangdong General Hospital, School of Medicine, South China University of Technology, Guangdong, China.

Abstract

Constructing the interconnected porous biomaterials scaffolds with osteogenesis and angiogenesis capacity is extremely important for efficient bone tissue engineering. Herein, we fabricated a bioactive micro-nano composite scaffolds with excellent in vitro osteogenesis and angiogenesis capacity, based on poly (lactic-co-glycolic acid) (PLGA) incorporated with micro-nano bioactive glass (MNBG). The results showed that the addition of MNBG enlarged the pore size, increased the compressive modulus (4 times improvement), enhanced the physiological stability and apatite-forming ability of porous PLGA scaffolds. The in vitro studies indicated that the PLGA-MNBG porous scaffold could enhance the mouse bone mesenchymal stem cells (mBMSCs) attachment, proliferation, and promote the expression of osteogenesis marker (ALP). Additionally, PLGA-MNBG could also support the attachment and proliferation of human umbilical vein endothelial cells (HUVECs), and significantly enhanced the expression of angiogenesis marker (CD31) of HUVECs. The as-prepared bioactive PLGA-MNBG nanocomposites scaffolds with good osteogenesis and angiogenesis probably have a promising application for bone tissue regeneration.

Keywords: bioactive glass; bone regeneration; micro-nano particles; nanocomposites scaffolds; osteogenesis.