YTOX: a rapid toxicity test based on the dehydrogenase activity of Saccharomyces cerevisiae for detection of contaminants in water samples

J Microbiol Methods. 2019 Jun:161:43-46. doi: 10.1016/j.mimet.2019.04.008. Epub 2019 Apr 15.

Abstract

A simple generic toxicity method (test) is proposed using baker's yeast to mediate the reduction of the colourless triphenyltetrazolium chloride (TTC) to red, 1,3,5-triphenyl formazan, which can be extracted by dimethyl sulfoxide (DMSO), enabling the identification of reducible toxic compounds (e.g. cadmium, fipronil) in water for consumption.

Keywords: Baker's yeast; Cadmium; Fipronil; Formazan; Gasoline; Methanol; TTC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadmium / analysis
  • Dimethyl Sulfoxide
  • Formazans
  • Gasoline
  • Oxidoreductases / metabolism*
  • Pyrazoles / analysis
  • Saccharomyces cerevisiae / metabolism*
  • Tetrazolium Salts
  • Toxicity Tests / methods*
  • Water
  • Water Pollutants, Chemical / analysis*

Substances

  • 1,3,5-triphenylformazan
  • Formazans
  • Gasoline
  • Pyrazoles
  • Tetrazolium Salts
  • Water Pollutants, Chemical
  • Cadmium
  • Water
  • triphenyltetrazolium
  • Oxidoreductases
  • fipronil
  • Dimethyl Sulfoxide