Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03

Chung Hong Tan; Pau-Loke Show; Tau Chuan Ling; Dillirani Nagarajan; Duu-Jong Lee; Wei-Hsin Chen; Jo-Shu Chang

doi:10.1016/j.biortech.2019.121331

Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03

Bioresour Technol. 2019 Aug:285:121331. doi: 10.1016/j.biortech.2019.121331. Epub 2019 Apr 11.

Authors

Chung Hong Tan¹, Pau-Loke Show², Tau Chuan Ling³, Dillirani Nagarajan⁴, Duu-Jong Lee⁵, Wei-Hsin Chen⁶, Jo-Shu Chang⁷

Affiliations

¹ Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
² Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia. Electronic address: [email protected].
³ Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁴ Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
⁵ Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
⁶ Department of Aeronautics and Astronautics, National Cheng Kung University, Tainan, Taiwan; Research Center for Circular Economy, National Cheng Kung University, Tainan 701, Taiwan.
⁷ Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Circular Economy, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan; College of Engineering, Tunghai University, Taichung 407, Taiwan.

PMID: 30999192
DOI: 10.1016/j.biortech.2019.121331

Abstract

Third generation biofuels, also known as microalgal biofuels, are promising alternatives to fossil fuels. One attractive option is microalgal biodiesel as a replacement for diesel fuel. Chlamydomonas sp. Tai-03 was previously optimized for maximal lipid production for biodiesel generation, achieving biomass growth and productivity of 3.48 ± 0.04 g/L and 0.43 ± 0.01 g/L/d, with lipid content and productivity of 28.6 ± 1.41% and 124.1 ± 7.57 mg/L/d. In this study, further optimization using 5% CO₂ concentration and semi-batch operation with 25% medium replacement ratio, enhanced the biomass growth and productivity to 4.15 ± 0.12 g/L and 1.23 ± 0.02 g/L/d, with lipid content and productivity of 19.4 ± 2.0% and 239.6 ± 24.8 mg/L/d. The major fatty acid methyl esters (FAMEs) were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). These short-chain FAMEs combined with high growth make Chlamydomonas sp. Tai-03 a suitable candidate for biodiesel synthesis.

Keywords: Biodiesel; Medium replacement; Microalgae; Organic carbon; Semi-batch.

MeSH terms

Biofuels
Biomass
Chlamydomonas*
Fatty Acids
Lipids
Microalgae*

Substances

Biofuels
Fatty Acids
Lipids