Generation of induced pluripotent stem cell line-NTUHi001-A from a premature ovarian failure patient with Turner's syndrome mosaicism

Chen-Yu Lu; Yu-An Chen; Shih-Han Syu; Huai-En Lu; Hong-Nerng Ho; Hsin-Fu Chen

doi:10.1016/j.scr.2019.101422

Generation of induced pluripotent stem cell line-NTUHi001-A from a premature ovarian failure patient with Turner's syndrome mosaicism

Stem Cell Res. 2019 May:37:101422. doi: 10.1016/j.scr.2019.101422. Epub 2019 Apr 2.

Authors

Chen-Yu Lu¹, Yu-An Chen¹, Shih-Han Syu², Huai-En Lu², Hong-Nerng Ho³, Hsin-Fu Chen⁴

Affiliations

¹ Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan.
² Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan.
³ Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei, Taiwan.
⁴ Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei, Taiwan. Electronic address: [email protected].

PMID: 31004936
DOI: 10.1016/j.scr.2019.101422

Abstract

Turner's syndrome (TS) is one of the main causes of premature ovarian failure (POF). However, the mechanisms underlying POF are difficult to study due to the lack of suitable disease models. Herein, we have generated a human induced pluripotent stem cell (hiPSC) line derived from the peripheral blood mononuclear cells of a female patient with Turner's syndrome mosaicism via integration-free Sendai-virus system. The hiPSCs were confirmed with a 45, X karyotype and the acquisition of pluripotency. It's likely that hiPSCs can serve as a feasible cellular model for further pathophysiological studies of POF cases, especially for those originating in TS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation*
Cells, Cultured
Cellular Reprogramming*
Female
Humans
Induced Pluripotent Stem Cells / metabolism
Induced Pluripotent Stem Cells / pathology*
Leukocytes, Mononuclear / metabolism
Leukocytes, Mononuclear / pathology*
Mosaicism
Phenotype
Primary Ovarian Insufficiency / complications
Primary Ovarian Insufficiency / genetics
Primary Ovarian Insufficiency / pathology*
Turner Syndrome / complications
Turner Syndrome / genetics
Turner Syndrome / pathology*