Allicin inhibits Pseudomonas aeruginosa virulence by suppressing the rhl and pqs quorum-sensing systems

Can J Microbiol. 2019 Aug;65(8):563-574. doi: 10.1139/cjm-2019-0055. Epub 2019 Apr 22.

Abstract

Pseudomonas aeruginosa is a virulent bacterium that secretes a variety of virulence factors that aid in establishing infections in individuals. Allicin, derived from garlic, has been shown to inhibit virulence factor production and biofilm formation in P. aeruginosa. However, the mechanisms underlying the allicin-mediated regulation of P. aeruginosa virulence remain unclear. In this study, we investigated the possible mechanisms underlying allicin-mediated virulence regulation in P. aeruginosa. The results showed that allicin attenuates the production of P. aeruginosa virulence-associated factors, such as elastase, pyocyanin, pyoverdine, and rhamnolipids, by inhibiting the rhl and pqs quorum-sensing systems. Further analysis revealed that the rhl and pqs systems play different roles during the allicin-mediated regulation process. Taken together, these results support the potential use of allicin as a therapeutic agent in controlling P. aeruginosa infection and associated mechanisms.

Keywords: allicin; allicine; facteurs de virulence; quorum-sensing system; système de détection de quorum; virulence factors.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Disulfides
  • Gene Expression Regulation, Bacterial / drug effects
  • Glycolipids / metabolism
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / metabolism
  • Pseudomonas aeruginosa / pathogenicity*
  • Pyocyanine / metabolism
  • Quorum Sensing / drug effects*
  • Sulfinic Acids / pharmacology*
  • Virulence / drug effects
  • Virulence Factors / genetics
  • Virulence Factors / metabolism*

Substances

  • Bacterial Proteins
  • Disulfides
  • Glycolipids
  • Sulfinic Acids
  • Virulence Factors
  • rhamnolipid
  • allicin
  • Pyocyanine