Dendrobium candidum protects against diabetic kidney lesions through regulating vascular endothelial growth factor, Glucose Transporter 1, and connective tissue growth factor expression in rats

J Cell Biochem. 2019 Aug;120(8):13924-13931. doi: 10.1002/jcb.28666. Epub 2019 Apr 25.

Abstract

Diabetes mellitus (DM) remains a great health problem with approximate 30% of patients with DM eventually suffering from diabetic nephropathy. The search for exogenous protective factors has recently received wide attention. The current study aimed to investigate the protective effects of Dendrobium candidum (DC) on kidneys in diabetic rats. Initially, streptozotocin-induced diabetic rats were established and randomly divided into the model group, DC group (0.2, 0.4, and 0.8 g/kg) and irbesartan group (17.5 mg/kg). The biochemical indexes, pathological changes, and the expressions of vascular endothelial growth factor (VEGF), GLUT-1, and CTGF were examined. It was found that as compared with the model group, the kidney index, serum creatine, blood urea nitrogen, 24-hour urine protein, and VEGF of DC treatment groups were significantly decreased, and pathological changes in kidney were improved in the DC groups and irbesartan group ( P < 0.05 for each parameter). The protein and messenger RNA levels of GLUT-1 and CTGF in treatment groups were significantly lower than those in rats' renal cortex without treatment. Our data suggest that DC may protect the kidneys of diabetic rats via regulating expression of VEGF, GLUT-1, and CTGF.

Keywords: CTGF; Dendrobium candidum; GLUT-1; diabetic nephropathy; vascular endothelial growth factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Connective Tissue Growth Factor / genetics
  • Connective Tissue Growth Factor / metabolism*
  • Dendrobium / chemistry*
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / genetics
  • Diabetes Mellitus, Experimental / pathology*
  • Diabetes Mellitus, Experimental / physiopathology
  • Glucose Transporter Type 1 / genetics
  • Glucose Transporter Type 1 / metabolism*
  • Kidney / drug effects
  • Kidney / pathology*
  • Kidney / physiopathology
  • Kidney Cortex / drug effects
  • Kidney Cortex / pathology
  • Male
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Protective Agents / pharmacology
  • Protective Agents / therapeutic use
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Vascular Endothelial Growth Factor A / metabolism*

Substances

  • Glucose Transporter Type 1
  • Plant Extracts
  • Protective Agents
  • RNA, Messenger
  • Vascular Endothelial Growth Factor A
  • Connective Tissue Growth Factor