FOXO3a knockdown promotes radioresistance in nasopharyngeal carcinoma by inducing epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway

Min Luo; Cheng Wu; Ergang Guo; Shan Peng; Linli Zhang; Wei Sun; Dongbo Liu; Guangyuan Hu; Guoqing Hu

doi:10.1016/j.canlet.2019.04.019

FOXO3a knockdown promotes radioresistance in nasopharyngeal carcinoma by inducing epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway

Cancer Lett. 2019 Jul 28:455:26-35. doi: 10.1016/j.canlet.2019.04.019. Epub 2019 Apr 28.

Authors

Min Luo¹, Cheng Wu², Ergang Guo², Shan Peng¹, Linli Zhang², Wei Sun², Dongbo Liu², Guangyuan Hu³, Guoqing Hu⁴

Affiliations

¹ Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, China.
² Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan, People's Republic of China.
³ Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan, People's Republic of China. Electronic address: [email protected].
⁴ Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan, People's Republic of China. Electronic address: [email protected].

PMID: 31022422
DOI: 10.1016/j.canlet.2019.04.019

Abstract

Mutations in the forkhead box O 3a (FOXO3a) gene are closely related to the progression of several types of cancers. However, few studies explore the relationship between FOXO3a and nasopharyngeal carcinoma (NPC). Our findings demonstrate that silencing FOXO3a promotes tumor radioresistance of NPC in vitro and in vivo through inducing EMT and activating Wnt/β-catenin signal pathway. These data establish that FOXO3a can be a novel and reliable NPC marker and a potential therapeutic target against NPC.

Keywords: Epithelial-mesenchymal transition; FOXO3a; NPC; Radioresistance; Wnt.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line, Tumor
Epithelial-Mesenchymal Transition
Female
Forkhead Box Protein O3 / deficiency
Forkhead Box Protein O3 / genetics
Forkhead Box Protein O3 / metabolism*
Gene Knockdown Techniques
Humans
Mice
Mice, Inbred BALB C
Nasopharyngeal Carcinoma / metabolism*
Nasopharyngeal Carcinoma / pathology
Nasopharyngeal Carcinoma / radiotherapy*
Nasopharyngeal Neoplasms / metabolism*
Nasopharyngeal Neoplasms / pathology
Nasopharyngeal Neoplasms / radiotherapy*
Radiation Tolerance
Random Allocation
Wnt Signaling Pathway*
beta Catenin / metabolism*

Substances

CTNNB1 protein, human
FOXO3 protein, human
Forkhead Box Protein O3
beta Catenin