CEL-Seq2-Single-Cell RNA Sequencing by Multiplexed Linear Amplification

Methods Mol Biol. 2019:1979:45-56. doi: 10.1007/978-1-4939-9240-9_4.

Abstract

Single-cell RNA sequencing has revolutionized the way we look at cell populations. Of the methods available, CEL-Seq was the first to use linear RNA amplification. With early barcoding and 3' sequencing, it is sensitive, cost-effective and easy to perform. Here we describe a protocol for performing CEL-Seq2 on sorted cells, which can be performed without any special equipment.

Keywords: 3′ sequencing; Gene expression; In vitro transcription; Single-cell RNA amplification; Single-cell transcriptomics.

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Complementary / genetics
  • Flow Cytometry
  • Gene Expression Profiling / economics
  • Gene Expression Profiling / methods*
  • Gene Library
  • Humans
  • Nucleic Acid Amplification Techniques / economics
  • Nucleic Acid Amplification Techniques / methods
  • RNA / genetics*
  • Sequence Analysis, RNA / economics
  • Sequence Analysis, RNA / methods*
  • Single-Cell Analysis / economics
  • Single-Cell Analysis / methods*

Substances

  • DNA, Complementary
  • RNA