Single-cell genome sequencing can detect low-frequency genetic alterations present in complex tissues. However, the experimental procedures are technically challenging. This includes dissociation of the tissue, isolation of single cells, whole-genome amplification, sequencing library preparation, and an optional target enrichment. Here we describe how to perform each of these processes to obtain high-quality single-cell genome sequencing data.
Keywords: DOP-PCR; Genomics; MDA; Sequencing library preparation; Single-cell isolation; Target enrichment; WGA; Whole-genome amplification (WGA).