Abstract
Proteins are often tagged for visualization or delivery in the "sea" of other macromolecules in cells but how tags affect protein mobility remains poorly understood. Here, we employ in-cell 19F NMR to quantify the mobility of proteins with charged tags in Escherichia coli cells and Xenopus laevis oocytes. We find that the transient charge-charge interactions between the tag and cellular components affect protein mobility. More specifically, positively charged tags impede protein mobility.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism*
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Diffusion*
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Escherichia coli
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Fluorine / chemistry
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / metabolism*
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Nuclear Magnetic Resonance, Biomolecular
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Streptococcus / chemistry
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Ubiquitins / chemistry
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Ubiquitins / metabolism*
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Xenopus laevis
Substances
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Bacterial Proteins
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IgG Fc-binding protein, Streptococcus
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Ubiquitins
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Green Fluorescent Proteins
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Fluorine