RNA chaperone hfq mediates persistence to multiple antibiotics in Aeromonas veronii

Microb Pathog. 2019 Jul:132:124-128. doi: 10.1016/j.micpath.2019.04.045. Epub 2019 May 1.

Abstract

Pathogenic Aeromonas veronii results in great healthy and economic losses in fishes and human. The multiple drug tolerance of bacterial persister is the major cause for recurrent infections. Ubiquitous RNA-binding protein Hfq is liable for antibiotic tolerance and persisiter production. We showed that the hfq deletion in A. veronii retarded the growth, reduced the tolerances to diverse antibiotics, and lowered the persistence. Such effects might be mediated by the downregulations of RelE, CspD, ClpB, RpoS, OxyR, and upregulation of OppB. Our study supports the role of Hfq in persister formation and provides clues for the avoidance of recalcitrant infections.

Keywords: Aeromonas veronii; Hfq; Persisters; Resistance; growth.

MeSH terms

  • Aeromonas veronii / drug effects
  • Aeromonas veronii / genetics*
  • Aeromonas veronii / growth & development*
  • Anti-Bacterial Agents / pharmacology*
  • Drug Resistance, Bacterial / genetics
  • Gene Deletion
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial / genetics
  • Host Factor 1 Protein / genetics*
  • Host Factor 1 Protein / physiology*
  • Microbial Sensitivity Tests
  • RNA
  • Transcriptome
  • Virulence / genetics

Substances

  • Anti-Bacterial Agents
  • Host Factor 1 Protein
  • RNA