Adipogenesis induces growth inhibition of dedifferentiated liposarcoma

Cancer Sci. 2019 Aug;110(8):2676-2683. doi: 10.1111/cas.14036. Epub 2019 Jun 28.

Abstract

Well-differentiated liposarcoma (WDLPS) and dedifferentiated liposarcoma (DDLPS) are the most common types of liposarcoma. Although WDLPS and DDLPS patients receive intensive treatment including radical surgery and systemic therapy, their overall 5-year survival rates are 90% and 30%, respectively, indicating that DDLPS is clinically more aggressive. We examined whether adipogenic stimulation induces adipogenesis in human WDLPS/DDLPS cells by using dexamethasone, indomethacin, insulin, and 3-isobutyl-1-methylxanthine (IBMX), all putative medications or drugs. Functional in vitro experiments showed that treatment with these four compounds induced adipogenic potency by transcriptional and translational upregulation of genes related to the maintenance of stemness and adipogenic differentiation. Using in vivo xenograft models, we found that the induction of stemness and adipogenesis inhibited the tumorigenic potency of DDLPS. This study suggests a potential application of drug repositioning in which adipogenesis-inducing compounds could be used to treat DDLPS patients in a clinical setting.

Keywords: adipogenesis; dedifferentiated liposarcoma; stemness; well-differentiated liposarcoma; xenograft.

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology
  • Adipogenesis / drug effects
  • Adipogenesis / genetics*
  • Cell Dedifferentiation / drug effects
  • Cell Dedifferentiation / genetics*
  • Cell Differentiation / drug effects
  • Cell Differentiation / genetics
  • Cell Line, Tumor
  • Cell Proliferation / genetics*
  • Dexamethasone / pharmacology
  • Humans
  • Indomethacin / pharmacology
  • Insulin / pharmacology
  • Liposarcoma / drug therapy
  • Liposarcoma / genetics*
  • Liposarcoma / pathology
  • Protein Biosynthesis / drug effects
  • Protein Biosynthesis / genetics
  • Transcription, Genetic / drug effects
  • Transcription, Genetic / genetics
  • Up-Regulation / drug effects
  • Up-Regulation / genetics

Substances

  • Insulin
  • Dexamethasone
  • 1-Methyl-3-isobutylxanthine
  • Indomethacin