Water-soluble AIE-Active Fluorescent Organic Nanoparticles: Design, Preparation and Application for Specific Detection of Cysteine over Homocysteine and Glutathione in Living Cells

Chem Asian J. 2019 Jul 1;14(13):2220-2224. doi: 10.1002/asia.201900551. Epub 2019 Jun 6.

Abstract

Water-soluble ratiometric AIE-active fluorescent organic nanoparticles 2OA-FON for the specific sensing of cysteine over other biothiols are reported. The obtained amphiphilic probe included olefin aldehyde as recognizing unit, tetraphenylethylene as fluorescence reporter and lactose moiety as a hydrophilic group. This work provides a general design strategy based on the introduction of a sugar moiety into a hydrophobic AIEgen to develop ratiometric water-soluble fluorescent organic nanoparticles.

Keywords: aggregation-induced emission; biothiols; cysteine; nanoparticles; tetraphenylethylene.

MeSH terms

  • Cysteine / analysis*
  • Dimerization
  • Fluorescent Dyes / chemistry*
  • Glutathione / analysis*
  • Hep G2 Cells
  • Homocysteine / analysis*
  • Humans
  • Microscopy, Confocal
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Optical Imaging
  • Solubility
  • Spectrometry, Fluorescence
  • Stilbenes / chemistry*
  • Water / chemistry

Substances

  • Fluorescent Dyes
  • Stilbenes
  • tetraphenylethylene
  • Water
  • Homocysteine
  • Glutathione
  • Cysteine