Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139

Hidemasa Izumiya; Masatomo Morita; Eiji Arakawa; Tuan Cuong Ngo; Hoai Thu Nguyen; Dong Tu Nguyen; Makoto Ohnishi

doi:10.1016/j.mcp.2019.05.001

Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139

Mol Cell Probes. 2019 Jun:45:65-67. doi: 10.1016/j.mcp.2019.05.001. Epub 2019 May 10.

Authors

Hidemasa Izumiya¹, Masatomo Morita², Eiji Arakawa², Tuan Cuong Ngo³, Hoai Thu Nguyen³, Dong Tu Nguyen³, Makoto Ohnishi²

Affiliations

¹ Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: [email protected].
² Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan.
³ Department of Bacteriology, National Institute for Hygiene and Epidemiology, Hanoi, Viet Nam.

PMID: 31082474
DOI: 10.1016/j.mcp.2019.05.001

Abstract

A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5-67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.

Keywords: Cholera; LAMP; O1/O139.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Cholera / diagnosis
Environmental Monitoring
Humans
Limit of Detection
Molecular Diagnostic Techniques / methods*
Nucleic Acid Amplification Techniques / methods*
Vibrio cholerae O1 / genetics
Vibrio cholerae O1 / isolation & purification*
Vibrio cholerae O139 / genetics
Vibrio cholerae O139 / isolation & purification*

Substances

Bacterial Proteins
RfbN protein, Vibrio cholerae

Supplementary concepts

LAMP assay