Effects of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in a human cervical cancer cell line

Weiguo Song; Juan Wang; Hanyuan Liu; Chenchen Zhu; Fei Xu; Lili Qian; Zhen Shen; Jing Zhu; Shuai Yin; Jiwei Qin; Liang Chen; Dabao Wu; Björn Nashan; Ge Shan; Weihua Xiao; Ying Zhou

doi:10.1016/j.cyto.2019.05.004

Effects of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in a human cervical cancer cell line

Cytokine. 2019 Aug:120:165-175. doi: 10.1016/j.cyto.2019.05.004. Epub 2019 May 11.

Authors

Weiguo Song¹, Juan Wang¹, Hanyuan Liu¹, Chenchen Zhu¹, Fei Xu¹, Lili Qian², Zhen Shen², Jing Zhu², Shuai Yin³, Jiwei Qin³, Liang Chen⁴, Dabao Wu², Björn Nashan³, Ge Shan⁵, Weihua Xiao⁶, Ying Zhou⁷

Affiliations

¹ Department of Obstetrics and Gynecology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China.
² Department of Obstetrics and Gynecology, The First Affiliated Hospital of University of Science & Technology of China, Anhui Provincial Hospital, Hefei, Anhui Province 230001, China.
³ Organ Transplantation Center, The First Affiliated Hospital of University of Science & Technology of China, Anhui Provincial Hospital, Hefei 230001, China.
⁴ Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
⁵ Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China. Electronic address: [email protected].
⁶ Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China. Electronic address: [email protected].
⁷ Department of Obstetrics and Gynecology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China. Electronic address: [email protected].

PMID: 31085454
DOI: 10.1016/j.cyto.2019.05.004

Abstract

This study explored the effect of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in the human cervical cancer (HCC) cell line SiHa. SiHa cells had the lowest expression of Lnc-LIF-AS in the 4 human cervical cancer cell lines (SiHa, ME-180, C-33A and HeLa) and were transfected and divided into the SiHa/con (transfected with pMIGRI) cell group, SiHa/Lnc-LIF-AS (transfected with pMIGRI-Lnc-LIF-AS) cell group, and SiHa/Lnc-LIF-AS-DN (transfected with pMIGRI-Lnc-LIF-AS-DN, in which the sequences overlapping with LIF mRNA was deleted) cell group. Overexpression of Lnc-LIF-AS could promote the proliferation, colony formation, invasion and migration in SiHa and ME-180 cells. And the low expression of Lnc-LIF-AS suppress the proliferation, colony formation invasion and migration in HeLa cells when the Lnc-LIF-AS expression has been suppressed. In the SiHa/Lnc-LIF-AS cells group, the cell cycle was mainly halted in the S phase and overexpression of Lnc-LIF-AS had no effect on the apoptosis of SiHa cells. Overexpression of Lnc-LIF-AS could promote the secretion of LIF in SiHa cells, and the supernatant from SiHa/Lnc-LIF-AS cells could promote cell proliferation in the SiHa/con cells. The STAT3 inhibitor could inhibit cell proliferation in the SiHa/Lnc-LIF-AS cells. The expression level of Lnc-LIF-AS in cervical cancer tissues was higher than that in normal tissues and the expression level of Lnc-LIF-AS was positively correlated with the level of LIF. In the SiHa/con and SiHa/Lnc-LIF-AS-DN cell groups, there were no significant differences in cell proliferation, cell migration and cell invasion. The overexpression of Lnc-LIF-AS can promote cell proliferation, migration and invasion in cervical cancer cells, and the core function domain of this lncRNA was located in the overlapping a 3'-UTR base sequence of LIF mRNA.

Keywords: Cervical cancer; Invasion; LncRNA; Migration; Proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions / genetics
Animals
Base Sequence
Cell Line, Tumor
Cell Movement / genetics*
Cell Proliferation / genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Mice, Nude
MicroRNAs / genetics
MicroRNAs / metabolism
Neoplasm Invasiveness
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Uterine Cervical Neoplasms / genetics*
Uterine Cervical Neoplasms / pathology*

Substances

3' Untranslated Regions
MicroRNAs
RNA, Long Noncoding
RNA, Messenger