Two independent domains of factor VIII co-expressed using recombinant vaccinia viruses have procoagulant activity

Biochem Biophys Res Commun. 1987 May 29;145(1):234-40. doi: 10.1016/0006-291x(87)91311-8.

Abstract

Using recombinant DNA technology, the NH2 and COOH terminal domains of the human Factor VIII molecule were co-expressed in baby hamster kidney 21 (BHK21) cells using the vaccinia virus system. Procoagulant activity was detectable in cell supernatants, thus suggesting that the central portion present in the FVIII protein (domain B) is not required for FVIII function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cloning, Molecular
  • Factor VIII / genetics*
  • Factor VIII / metabolism
  • Genetic Vectors
  • Humans
  • Plasmids
  • Recombinant Proteins / metabolism*
  • Vaccinia virus / genetics*

Substances

  • Recombinant Proteins
  • Factor VIII