Unique Inflammatory Changes in Exocrine and Endocrine Pancreas in Enterovirus-Induced Fulminant Type 1 Diabetes

J Clin Endocrinol Metab. 2019 Oct 1;104(10):4282-4294. doi: 10.1210/jc.2018-02672.

Abstract

Context: There are scant reports on the pathological changes of the exocrine and endocrine pancreas in fulminant type 1 diabetes mellitus (FT1DM).

Objective: To clarify the distinct pathological changes in the exocrine as well as the endocrine pancreas shortly after onset of diabetes in FT1DM.

Design: The exocrine and endocrine pancreases of 3 patients with FT1DM and 17 nondiabetic controls were immunohistochemically examined for islet and exocrine tissue inflammation, infiltrating mononuclear cell (MNC) CD subtype, enterovirus capsid protein 1 (VP1) localization, and CXC chemokine ligand 10 (CXCL10) and CXC chemokine receptor 3 (CXCR3) expressions.

Results: The median frequency of insulitis in the 3 FT1DM pancreases was 60%. In the nondiabetic control pancreases, no insulitis was observed. In the islets of FT1DM, the numbers of CD45+, CD3+, CD8+, CD68+, and CD11c+ MNCs were significantly higher than those of the control group. In the exocrine pancreas of FT1DM, the numbers of CD3+ T cells, CD8+ T cells, CD68+ macrophages, and CD11c+ dendritic cells were significantly higher than those of the control group. Infiltrating CD8+ T cells, CD68+ macrophages, and CD11c+ dendritic cells were observed around exocrine acinar cells in FT1DM. There was a close association between VP1 and CXCL10 expression in pancreatic exocrine ductal cells and acinar cells as well as islet cells in FT1DM. CXCL10+ exocrine cells were surrounded by CXCR3+ T cells.

Conclusion: The pathological findings suggested that suppression of the activated CXCL10-CXCR3 axis in the exocrine as well as the endocrine pancreas is a novel therapeutic target in FT1DM and possibly in enterovirus-associated acute-onset type 1 diabetes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomarkers / metabolism
  • Biopsy, Needle
  • CD11 Antigens / metabolism
  • Capsid Proteins / metabolism
  • Diabetes Mellitus, Type 1 / etiology
  • Diabetes Mellitus, Type 1 / pathology*
  • Diabetes Mellitus, Type 1 / virology*
  • Enterovirus / isolation & purification
  • Enterovirus Infections / complications*
  • Female
  • Hospitals, University
  • Humans
  • Immunohistochemistry
  • Islets of Langerhans / pathology*
  • Japan
  • Male
  • Middle Aged
  • Pancreas, Exocrine / pathology*
  • Pancreatitis, Acute Necrotizing / pathology*
  • Pancreatitis, Acute Necrotizing / virology
  • Receptors, Interleukin-8A / metabolism
  • Retrospective Studies
  • Sampling Studies
  • Sensitivity and Specificity
  • Severity of Illness Index
  • Young Adult

Substances

  • Biomarkers
  • CD11 Antigens
  • Capsid Proteins
  • Receptors, Interleukin-8A
  • VP1 protein, enterovirus B