Whole-genome analysis of New Delhi Metallo-Beta-Lactamase-1-producing Acinetobacter haemolyticus from China

Lili Jiang; Yunmei Yu; Wei Zeng; Jiafan Guo; Fei Lv; Xin Wang; Xiaoliu Liu; Zuguo Zhao

doi:10.1016/j.jgar.2019.05.012

Whole-genome analysis of New Delhi Metallo-Beta-Lactamase-1-producing Acinetobacter haemolyticus from China

J Glob Antimicrob Resist. 2020 Mar:20:204-208. doi: 10.1016/j.jgar.2019.05.012. Epub 2019 May 18.

Authors

Lili Jiang¹, Yunmei Yu², Wei Zeng³, Jiafan Guo¹, Fei Lv¹, Xin Wang¹, Xiaoliu Liu⁴, Zuguo Zhao⁵

Affiliations

¹ Department of Microbiology and Immunology of Guangdong Medical University, Zhanjiang, Guangdong Province, China.
² Clinical lab of 422nd Center Hospital of the People' Liberation Army, Zhanjiang, Guangdong Province, China.
³ Department of Neurology, Fifth Hospital (Second Hospital Affiliated to Jianghan University) of Wuhan City, Wuhan, China.
⁴ Medical Laboratory of ShenZhen LuoHu People's Hospital, Shenzhen, Guangdong Province, China.
⁵ Department of Microbiology and Immunology of Guangdong Medical University, Zhanjiang, Guangdong Province, China. Electronic address: [email protected].

PMID: 31112806
DOI: 10.1016/j.jgar.2019.05.012

Abstract

Objective: Infections caused by multidrug-resistant Acinetobacter spp. have generated worldwide attention. With the increasing isolation of non-baumannii Acinetobacter, the nature of associated infection and resistance needs to be explored. This study aimed to analyse the characteristics of New Delhi Metallo-Beta-Lactamase-1 (NDM-1)-producing Acinetobacter haemolyticus (named sz1652) isolated from Shenzhen city, China.

Methods: The antibiotic spectrum was analysed after antimicrobial susceptibility testing. Combined disk test (CDT) was used to detect the metallo-beta-lactamases (MBLs). Transferability of carbapenem resistance was tested by filter mating experiments and plasmid transformation assays. Whole-genome sequencing (WGS) was performed using HiSeq 2000 and PacBio RS system.

Results: The Acinetobacter haemolyticus strain sz1652 was resistant to carbapenems and other tested agents except for amikacin, tigecycline and colistin. Production of MBLs was confirmed by CDT. Transfer of carbapenem resistance was unsuccessful. WGS analysis showed that the genome of sz1652 comprised a chromosome and two plasmids; 16 genomic islands (GIs) were predicted. Genes associated with resistance were found in this strain, including the beta-lactamase genes bla_NDM-1, bla_OXA-214 and bla_LRA-18, the ﬂuoroquinolone resistant-related mutations [GyrA subunits (Ser81Ile) and ParC subunits (Ser84Tyr)], and efflux pump genes related to tetracycline and macrolide resistance. Analysis of the genetic environment showed that bla_NDM-1 was embedded in Tn125 transposon. The Tn125 structure was chromosomally located and shared > 99% sequence identity with the previously reported bla_NDM-1 carrying region.

Conclusion: The NDM-1-producing Acinetobacter haemolyticus coexisted with multiple drug-resistant determinants. The acquisition of the bla_NDM-1 gene was probably facilitated by Tn125 in this strain. Non-Acinetobacter baumannii species also contained GIs.

Keywords: Acinetobacter haemolyticus; Carbapenemase; Genomic islands; Resistome; bla(NDM-1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter / drug effects
Acinetobacter / genetics
Acinetobacter / isolation & purification*
Acinetobacter / metabolism
Anti-Bacterial Agents / pharmacology*
Base Composition
Carbapenems / pharmacology
China
DNA Transposable Elements
Drug Resistance, Bacterial
Genome Size
Genome, Bacterial
Genomic Islands
High-Throughput Nucleotide Sequencing
Humans
Microbial Sensitivity Tests
Urine / microbiology
Whole Genome Sequencing / methods*
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Carbapenems
DNA Transposable Elements
beta-Lactamases
beta-lactamase NDM-1

Supplementary concepts

Acinetobacter haemolyticus