Development of the Com1 synthetic peptide-based Latex Agglutination Test (LAT) and its comparative evaluation with commercial indirect-ELISA for sero-screening of coxiellosis in cattle

Manesh Kumar; Satyaveer Singh Malik; Jess Vergis; Sunitha Ramanjeneya; Radhakrishna Sahu; Richa Pathak; Jay Prakash Yadav; Pankaj Dhaka; Sukhadeo B Barbuddhe; Deepak B Rawool

doi:10.1016/j.mimet.2019.05.012

Development of the Com1 synthetic peptide-based Latex Agglutination Test (LAT) and its comparative evaluation with commercial indirect-ELISA for sero-screening of coxiellosis in cattle

J Microbiol Methods. 2019 Jul:162:83-85. doi: 10.1016/j.mimet.2019.05.012. Epub 2019 May 27.

Affiliations

¹ Division of Veterinary Public Health, ICAR- Indian Veterinary Research Institute, Izatnagar 243 122, India.
² ICAR- National Research Centre on Meat, Hyderabad 500 092, India.
³ Division of Veterinary Public Health, ICAR- Indian Veterinary Research Institute, Izatnagar 243 122, India. Electronic address: [email protected].

PMID: 31145941
DOI: 10.1016/j.mimet.2019.05.012

Abstract

A novel Com1 synthetic peptide-based latex agglutination test (LAT) was developed and evaluated against commercial ELISA kit for sero-screening of coxiellosis in cattle. The developed test is economical, has field applicability and can serve as an important rapid tool for sero-screening of coxiellosis in cattle.

Keywords: Com1; Coxiellosis; LAT; Synthetic-peptide.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cattle Diseases / diagnosis*
Coxiella burnetii / isolation & purification*
Enzyme-Linked Immunosorbent Assay / veterinary
Latex Fixation Tests / veterinary*
Mass Screening / veterinary*
Q Fever / diagnosis*
Q Fever / veterinary*
Sensitivity and Specificity