On-line rapid purification of [13N]N2 gas for visualization of nitrogen fixation and translocation in nodulated soybean

Yong-Gen Yin; Satomi Ishii; Nobuo Suzui; Masato Igura; Keisuke Kurita; Yuta Miyoshi; Naotsugu Nagasawa; Mitsumasa Taguchi; Naoki Kawachi

doi:10.1016/j.apradiso.2019.05.034

On-line rapid purification of [¹³N]N₂ gas for visualization of nitrogen fixation and translocation in nodulated soybean

Appl Radiat Isot. 2019 Sep:151:7-12. doi: 10.1016/j.apradiso.2019.05.034. Epub 2019 May 28.

Authors

Yong-Gen Yin¹, Satomi Ishii², Nobuo Suzui², Masato Igura², Keisuke Kurita², Yuta Miyoshi³, Naotsugu Nagasawa², Mitsumasa Taguchi², Naoki Kawachi²

Affiliations

¹ Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), Takasaki, Gunma, 370-1292, Japan. Electronic address: [email protected].
² Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), Takasaki, Gunma, 370-1292, Japan.
³ Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), Takasaki, Gunma, 370-1292, Japan; Japan Society for the Promotion of Science, Japan.

PMID: 31151049
DOI: 10.1016/j.apradiso.2019.05.034

Abstract

Accurate analysis of N fixation in leguminous crops requires determination of N utilization within an intact plant; however, most approaches require tissue disassembly. We developed a simple and rapid technique to generate high-purity and high-yield [¹³N]N₂ gas and obtained real-time images of N fixation in an intact soybean plant. The purification efficiency was ∼81.6% after decay correction. Our method provides accurate signals of N fixation and allows free changes to the tracer gas composition to suit different experimental designs.

Keywords: Nitrogen fixation; Nodule; PETIS; Purification; [(13)N]N(2).

MeSH terms

Biological Transport
Chromatography, Gas
Crops, Agricultural / metabolism*
Glycine max / metabolism*
Nitrogen / metabolism*
Nitrogen Fixation*
Nitrogen Radioisotopes / isolation & purification*

Substances

Nitrogen Radioisotopes
Nitrogen