Esophageal cancer related gene-4 inhibits the migration and proliferation of oral squamous cell carcinoma through BC200 lncRNA/MMP-9 and -13 signaling pathway

Wenjun Huang; Rui Zhou; Liang Mao; Chenliang Deng; Xitong Dang

doi:10.1016/j.cellsig.2019.05.012

Esophageal cancer related gene-4 inhibits the migration and proliferation of oral squamous cell carcinoma through BC200 lncRNA/MMP-9 and -13 signaling pathway

Cell Signal. 2019 Oct:62:109327. doi: 10.1016/j.cellsig.2019.05.012. Epub 2019 May 29.

Authors

Wenjun Huang¹, Rui Zhou¹, Liang Mao¹, Chenliang Deng², Xitong Dang³

Affiliations

¹ The Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan 646000, China.
² Department of Plastic Surgery, Shanghai 6th People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200237, China. Electronic address: [email protected].
³ The Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan 646000, China. Electronic address: [email protected].

PMID: 31152845
DOI: 10.1016/j.cellsig.2019.05.012

Abstract

Esophageal cancer related gene-4 (ECRG4) inhibits the malignant phenotype of oral squamous cell carcinoma. However, the molecular mechanisms remain to be explored. Using the tongue carcinoma cell line, TCA8113 as a cell model, we showed that forced expression of ECRG4 down-regulated the expression of the BC200 long non-coding RNA (lncRNA) and matrix metalloproteinases (MMP-9 and MMP-13). Restoration of BC200 lncRNA rescued ECRG4-mediated down-regulation of MMP-9 and -13. Furthermore, over-expression of Ecrg4 inhibited cell proliferation and migration, which was abolished by forced expression of BC200 lncRNA in TCA8113 cells. Our results indicate that ECRG4 inhibits the malignant phenotype of TCA8113 cells most likely through suppression of BC200 lncRNA/MMPs signaling pathway, rationalizing that BC200 lncRNA may be a potential target for oral squamous cell carcinoma (OSCC) therapy.

Keywords: BC200 lncRNA; Cell migration; Cell proliferation; Esophageal cancer related gene-4; MMPs; Tumor suppressor gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / genetics
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Gene Expression Regulation, Neoplastic / genetics
Humans
Matrix Metalloproteinase 13 / genetics
Matrix Metalloproteinase 9 / genetics
Mouth Neoplasms / genetics*
Mouth Neoplasms / pathology
RNA, Long Noncoding / genetics*
Signal Transduction / genetics
Tumor Suppressor Proteins / genetics*

Substances

ECRG4 protein, human
RNA, Long Noncoding
Tumor Suppressor Proteins
long non-coding RNA BC200, human
Matrix Metalloproteinase 13
Matrix Metalloproteinase 9