Crystallization and preliminary X-ray study of pig lens aldose reductase

J Mol Biol. 1987 Jun 20;195(4):945-8. doi: 10.1016/0022-2836(87)90498-0.

Abstract

Crystals of pig lens aldose reductase have been grown from polyethylene glycol solutions at pH 6.2 and analysed by X-ray diffraction. Two crystal forms were obtained. The first belongs to space group P1 with unit cell dimensions a = 81.3 A, b = 85.9 A, c = 56.6 A, alpha = 102.3 degrees, beta = 103.3 degrees, gamma = 79.0 degrees, with four molecules in the unit cell related by a 222 non-crystallographic symmetry. The second crystal form is hexagonal. The space group is P6(2)22 with a = b = 101 A, c = 257 A and two molecules in the asymmetric unit. Both forms are suitable for X-ray structure analysis to better than 3 A resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehyde Reductase*
  • Animals
  • Crystallography
  • Lens, Crystalline / enzymology*
  • Sugar Alcohol Dehydrogenases*
  • Swine / metabolism*
  • X-Ray Diffraction

Substances

  • Sugar Alcohol Dehydrogenases
  • Aldehyde Reductase