First Molecular Diagnosis of Lophomoniasis: the End of a Controversial Story

Acta Parasitol. 2019 Jun;64(2):390-393. doi: 10.2478/s11686-019-00084-2. Epub 2019 Jun 5.

Abstract

Introduction: We report a case of lophomoniasis in an immunocompetent patient with acute paranasal sinusitis from the north of Iran whose disease was diagnosed by both microscopic and molecular methods.

Case presentation: The patient, a 40-year-old woman, suffered from upper respiratory infection, was referred to the Iranian National Registry Center for Lophomoniasis (INRCL) at the Mazandaran University of Medical Sciences, Sari, Iran, for diagnosis. A direct wet mount of nasal discharge revealed the flagellate protozoa morphologically identified Lophomonas blattarum. Moreover, through a specific polymerase chain reaction (PCR) of nasal discharge, a 214-bp band was observed, confirming the genus Lophomonas spp. The patient was treated successfully with metronidazole 500 mg t.i.d for 1 week.

Conclusion: To the best of our knowledge, this is the first molecular detection of lophomoniasis in the literature. According to our preliminary study, a reliable PCR test is available now for detecting the Lophomonas parasite.

Keywords: Human; Lophomonas; Molecular diagnosis; Sinusitis.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Antiprotozoal Agents / therapeutic use
  • Female
  • Humans
  • Immunocompetence
  • Iran
  • Lung Diseases, Parasitic / diagnosis*
  • Lung Diseases, Parasitic / parasitology
  • Metronidazole / therapeutic use
  • Molecular Diagnostic Techniques
  • Parabasalidea / genetics
  • Parabasalidea / isolation & purification*
  • Sinusitis / parasitology

Substances

  • Antiprotozoal Agents
  • Metronidazole