Melatonin effects on pulmonary tissue in the experimental model of Hepatopulmonary Syndrome

J Bras Pneumol. 2019 May 30;45(3):e20170164. doi: 10.1590/1806-3713/e20170164.
[Article in English, Portuguese]

Abstract

Objective: To evaluate the pulmonary alterations of animals with Hepatopulmonary Syndrome (HPS) submitted to Biliary Duct Ligature (BDL), as well as the antioxidant effect of Melatonin (MEL).

Methods: Sixteen male Wistar rats, divided into four Sham groups: BDL group, Sham + MEL group and BDL + MEL. The pulmonary and hepatic histology, lipoperoxidation and antioxidant activity of lung tissue, alveolar-arterial O2 difference and lung / body weight ratio (%) were evaluated.

Results: When comparing the groups, could be observed an increase of vasodilation and pulmonary fibrosis in the BDL group and the reduction of this in relation to the BDL + MEL group. It was also observed significant changes in the activity of catalase, ApCO2, ApO2 in the LBD group when compared to the other groups.

Conclusion: The use of MEL has been shown to be effective in reducing vasodilation, fibrosis levels and oxidative stress as well as gas exchange in an experimental HPS model.

Objetivo:: Avaliar as alterações pulmonares de animais com Síndrome Hepatopulmonar (SHP), submetidos à ligadura de ducto biliar (LDB), bem como o efeito antioxidante da Melatonina (MEL).

Métodos:: Dezesseis ratos machos da espécie Wistar, divididos em quatro grupos: Sham, Grupo LDB, Grupo Sham + MEL e LDB + MEL. Foram avaliadas a histologia pulmonar e hepática, a lipoperoxidação e atividade antioxidante do tecido pulmonar, diferença álveolo-arterial de O2 e relação peso pulmonar/peso corporal (%).

Resultados:: Quando comparados os grupos, observamos um aumento da vasodilatação e fibrose pulmonar no grupo LDB e a redução deste em relação ao grupo LDB+MEL. Observamos ainda alterações significativas na atividade da catalase, PaCO2, PaO2 no grupo LBD quando comparado aos demais grupos.

Conclusões:: A utilização da MEL demonstrou-se eficaz na redução da vasodilatação, níveis de fibrose e estresse oxidativo assim como na troca gasosa em modelo experimental de SHP.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Arterial Pressure / drug effects
  • Bile Ducts / surgery
  • Blood Gas Analysis
  • Catalase / analysis
  • Disease Models, Animal
  • Glutathione Transferase / analysis
  • Hepatopulmonary Syndrome / drug therapy*
  • Hepatopulmonary Syndrome / pathology
  • Hepatopulmonary Syndrome / physiopathology
  • Ligation
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / pathology
  • Liver / physiopathology
  • Liver Cirrhosis / drug therapy
  • Liver Cirrhosis / pathology
  • Liver Cirrhosis / physiopathology
  • Lung / drug effects*
  • Lung / pathology
  • Lung / physiopathology
  • Male
  • Melatonin / pharmacology*
  • Organ Size / drug effects
  • Oxidative Stress / drug effects
  • Rats, Wistar
  • Reproducibility of Results
  • Thiobarbituric Acid Reactive Substances / analysis
  • Treatment Outcome

Substances

  • Antioxidants
  • Thiobarbituric Acid Reactive Substances
  • Catalase
  • Glutathione Transferase
  • Melatonin